منابع مشابه
Release of hyaluronate from eukaryotic cells.
The mechanism of hyaluronate shedding from eukaryotic cell lines was analysed. All cell lines shed identical sizes of hyaluronate as were retained on the surface. They differed in the amount of hyaluronate synthesized and in the proportions of hyaluronate which were released and retained. A method was developed which could discriminate between shedding due to intramolecular degradation and that...
متن کاملIn Vitro Reconstitution of Eukaryotic Translation Reveals Cooperativity between Release Factors eRF1 and eRF3
Eukaryotic translation termination is triggered by peptide release factors eRF1 and eRF3. Whereas eRF1 recognizes all three termination codons and induces hydrolysis of peptidyl tRNA, eRF3's function remains obscure. Here, we reconstituted all steps of eukaryotic translation in vitro using purified ribosomal subunits; initiation, elongation, and termination factors; and aminoacyl tRNAs. This al...
متن کاملMutations in eukaryotic release factors 1 and 3 act as general nonsense suppressors in Drosophila.
In a screen for suppressors of the Drosophila wingless(PE4) nonsense allele, we isolated mutations in the two components that form eukaryotic release factor. eRF1 and eRF3 comprise the translation termination complex that recognizes stop codons and catalyzes the release of nascent polypeptide chains from ribosomes. Mutations disrupting the Drosophila eRF1 and eRF3 show a strong maternal-effect ...
متن کاملEthylene signaling via Ethylene Response Factors (ERFs) modifies wood development in hybrid aspen
Background The phytohormone ethylene (ET) has the potential to regulate secondary growth of plants and wood formation in trees. Application of exogenous ethylene or its in planta precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), to wood forming tissues of hybrid aspen (Populus tremula x Populus tremuloides) enhances xylem growth [1]. In the same study it was demonstrated that stimulation ...
متن کاملCombinatorial gene regulation by eukaryotic transcription factors.
Recent structure determinations of high order complexes of eukaryotic transcription factors bound to DNA have revealed that residues from their DNA-binding domains are involved in protein-protein interactions between distinct factors. Protein-protein interactions between transactivation domains and coactivators have also been characterized in a number of recently determined structures. These st...
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ژورنال
عنوان ژورنال: Biology of the Cell
سال: 2003
ISSN: 0248-4900
DOI: 10.1016/s0248-4900(03)00035-2